The AU-3S programme is driven by AUDA-NEPAD working with individuals from NRAs and EPIs of member states. The programme is technically supported by the UK Medicine and Health products Regulatory Agency (MHRA), the US Food and Drug Administration (FDA), WHO-AFRO, and WHO-EMRO. The AU-3S also works in close collaboration with other continental stakeholders such as Africa CDC, AMRH, and AVAREF. The Bill and Melinda Gates Foundation is the funding partner for AU-3S.

The MHRA is a key technical partner of the AU-3S programme. The MHRA is providing ongoing support and pharmacovigilance expertise to the AU-3S Joint Signal Management Group. The MHRA has also supported with implementing technological solutions, such as developing the AEFI reporting form for the Med Safety App and hosting the interim Data Integration and Signal Detection system. The MHRA further facilitated a series of training modules on end-to-end safety surveillance.